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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Plastin L antibody [LPL4A.1]
See all Plastin L products (7) ...
Description
Mouse monoclonal [LPL4A.1] to Plastin L
Specificity
This antibody does not react with T or I plastin. It recognizes LPL in Calcium independent fashion. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
IHC-Fr, IF, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Epitope
Headpiece region.
Positive control
Human polymorphonuclear neutrophils / monocytes, MCF 7, and Jurkat cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
L plastin is a leukocyte specific actin bundling protein from the fimbrin family of proteins. It has two actin binding domains and two EF hand type calcium binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L plastin may play a role in regulating integrin mediated adhesion in leukocytes. L plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosterone in androgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.
Clonality
Monoclonal
Clone number
LPL4A.1
Isotype
IgG1
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/50
IF: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Target
Function
Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Tissue specificity
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
Sequence similarities
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Post-translational
modifications
Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.
Cellular localization
Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
Target information above from: UniProt accessionP13796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- L plastin antibody
- L-plastin antibody
- LC64P antibody
see all
Database links
- Entrez Gene: 3936 Human
- Omim: 153430 Human
- SwissProt: P13796 Human
- Unigene: 381099 Human
- Unigene: 710563 Human
References for Anti-Plastin L antibody [LPL4A.1] (ab3290)
This product has been referenced in:
- Lollike Ket al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001).Read more (PubMed: 11279160) »
See 1 publication for this product
Publishing research using ab3290? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"