Overview

  • Product nameAnti-Plastin L antibody [LPL4A.1]
    See all Plastin L primary antibodies
  • Description
    Mouse monoclonal [LPL4A.1] to Plastin L
  • Specificityab80729 recognizes Plastin L in a Ca2+ independent fashion but does not react with T- or I- Plastin. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant Plastin L protein

  • EpitopeHeadpiece region of Plastin L
  • Positive control
    • Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.
IP Use at 2 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.

Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution.

Target

  • FunctionActin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • Tissue specificityDetected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • Sequence similaritiesContains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • Cellular localizationCytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP64 antibody
    • L plastin antibody
    • L-plastin antibody
    • Larval cuticle protein 1 antibody
    • LC64P antibody
    • LCP-1 antibody
    • LCP1 antibody
    • LPL antibody
    • Lplastin antibody
    • Lymphocyte cytosolic protein 1 antibody
    • Plastin 2 antibody
    • Plastin-2 antibody
    • PLS2 antibody
    • PLSL_HUMAN antibody
    see all

Anti-Plastin L antibody [LPL4A.1] images

  • Anti-Plastin L antibody [LPL4A.1] (ab80729) at 1 µg/ml + Human Cells-15 Lysate

    Predicted band size : 70 kDa
    Observed band size : 70 kDa

References for Anti-Plastin L antibody [LPL4A.1] (ab80729)

This product has been referenced in:
  • Lollike K  et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). Read more (PubMed: 11279160) »
  • Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996). Read more (PubMed: 8663066) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"