Overview

  • Product name
    Anti-Plastin L antibody [LPL4A.1]
    See all Plastin L primary antibodies
  • Description
    Mouse monoclonal [LPL4A.1] to Plastin L
  • Specificity
    ab80729 recognizes Plastin L in a Ca2+ independent fashion but does not react with T- or I- Plastin. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant Plastin L protein

  • Epitope
    Headpiece region of Plastin L
  • Positive control
    • Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab80729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.
IP Use at 2 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration.

Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution.

Target

  • Function
    Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • Tissue specificity
    Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
  • Sequence similarities
    Contains 2 actin-binding domains.
    Contains 4 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CP64 antibody
    • L plastin antibody
    • L-plastin antibody
    • Larval cuticle protein 1 antibody
    • LC64P antibody
    • LCP-1 antibody
    • LCP1 antibody
    • LPL antibody
    • Lplastin antibody
    • Lymphocyte cytosolic protein 1 antibody
    • Plastin 2 antibody
    • Plastin-2 antibody
    • PLS2 antibody
    • PLSL_HUMAN antibody
    see all

Anti-Plastin L antibody [LPL4A.1] images

  • Anti-Plastin L antibody [LPL4A.1] (ab80729) at 1 µg/ml + Human Cells-15 Lysate

    Predicted band size : 70 kDa
    Observed band size : 70 kDa

References for Anti-Plastin L antibody [LPL4A.1] (ab80729)

This product has been referenced in:
  • Lollike K  et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). Read more (PubMed: 11279160) »
  • Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996). Read more (PubMed: 8663066) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab80729.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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