Specificityab80729 recognizes Plastin L in a Ca2+ independent fashion but does not react with T- or I- Plastin.
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.
Use at 2 µg/mg of lysate.
Use at an assay dependent concentration.
Fixed in methanol or 3% paraformaldehyde. Use at an assay dependent dilution.
FunctionActin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Tissue specificityDetected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
Post-translational modificationsPhosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections. The N-terminus is blocked.
Cellular localizationCytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
Western blot - Plastin L antibody [LPL4A.1] - BSA and Azide free (ab80729)
Anti-Plastin L antibody [LPL4A.1] (ab80729) at 1 µg/ml + Human Cells-15 Lysate
Predicted band size : 70 kDa Observed band size : 70 kDa
References for Anti-Plastin L antibody [LPL4A.1] (ab80729)
This product has been referenced in:
Lollike K et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem276:17762-9 (2001).
Read more (PubMed: 11279160) »
Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem271:14623-30 (1996).
Read more (PubMed: 8663066) »