Platelet Glycoprotein IIb + IIIa Human ELISA Kit (ab108851)

Overview

  • Product namePlatelet Glycoprotein IIb + IIIa Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Plasma, Platelets, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1 ng/ml
  • Range
    1.25 ng/ml - 40 ng/ml
  • Recovery

    99 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelets, platelet-rich plasma and cell culture lysate.

    A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Platelet Glycoprotein IIb + IIIa Human ELISA Kit images

  • Representative Standard Curve using ab108851.

Protocols

References for Platelet Glycoprotein IIb + IIIa Human ELISA Kit (ab108851)

ab108851 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and interest in our products. I am very sorry for my delayed response.

I can confirm that the proper value for GPIIbIIIA in human plasma is about 600 ng/ml.

This kit will allow 1 x 96 tests and the price i...

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