Platelet Glycoprotein IIb + IIIa Human ELISA Kit (ab108851)
- Product namePlatelet Glycoprotein IIb + IIIa Human ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typePlasma, Platelets, Cell Lysate
- Assay typeSandwich (quantitative)
- Sensitivity= 1 ng/ml
- Range1.25 ng/ml - 40 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelets, platelet-rich plasma and cell culture lysate.
A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Biotinylated Human Platelet Glycoprotein IIb + IIIa Antibody 1 x 80µl 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 8ml Platelet Glycoprotein IIb + IIIa Microplate (12 x 8 well strips) 1 unit Platelet Glycoprotein IIb + IIIa Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
- Integrin alpha IIb beta 3
- Platelet membrane glycoprotein IIb/IIIa
Our Abpromise guarantee covers the use of ab108851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Platelet Glycoprotein IIb + IIIa Human ELISA Kit images
Representative Standard Curve using ab108851.
References for Platelet Glycoprotein IIb + IIIa Human ELISA Kit (ab108851)
ab108851 has not yet been referenced specifically in any publications.