The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 500 - 1000 µg/ml. Predicted molecular weight: 163 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PLB1 is a membrane-associated phospholipase which exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. It shows preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. It also exhibits esterase activity toward p-nitrophenyl. It may act on the brush border membrane to facilitate the absorption of digested lipids.
Apical cell membrane; Single-pass type I membrane protein. Note: Present in the intestinal brush border membranes.