Overview

  • Product name
  • Description
    Rabbit polyclonal to PLBD2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag, corresponding to amino acids 448-565 of PLBD2

  • Positive control
    • Human cerebellum

Properties

Applications

Our Abpromise guarantee covers the use of ab121526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Putative phospholipase.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in heart, brain and liver.
  • Sequence similarities
    Belongs to the phospholipase B-like family.
  • Post-translational
    modifications
    The p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form.
    Glycosylated; contains mannose 6-phosphate sugars.
  • Cellular localization
    Lysosome lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 76 kDa protein antibody
    • LAMA-like protein 2 antibody
    • Lamina ancestor homolog 2 antibody
    • p76 antibody
    • Phospholipase B domain-containing protein 2 antibody
    • Plbd2 antibody
    • PLBL2_HUMAN antibody
    • Putative phospholipase B-like 2 45 kDa form antibody
    see all

Images

  • Lane 1 :
    Lanes 2 - 3 : Anti-PLBD2 antibody (ab121526)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing PLBD2 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab121526 at 1/300 dilution staining PLBD2 in Paraffin-emebedded Human cerebellum tissue by Immunohistochemistry.

References

ab121526 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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