The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue specificityUbiquitously expressed, with highest levels in heart, brain and liver.
Sequence similaritiesBelongs to the phospholipase B-like family.
Post-translational modificationsThe p76 protein is synthesized as a 80 kDa precursor which is then processed into a N-terminal 32 kDa form and a C-terminal 45 kDa form. Glycosylated; contains mannose 6-phosphate sugars.