Overview

  • Product name
  • Description
    Rabbit polyclonal to PLC-delta-3
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: CDHSNNDRLE GAEIEEFLRR LLKRPELEEI FHQYSGEDRV LSAPELLEFL EDQGEEGATL ARAQQLIQTY ELNETAKQHE LMTLDGFMMY LL, corresponding to internal sequence amino acids 230-321 of Human PLC-delta-3.

  • Positive control
    • Human liver tissue; RT 4 and U 251 MG cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow.
  • Tissue specificity
    Present in corneal epithelial cells (at protein level).
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 3 EF-hand domains.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Domain
    The C2 domain is a Ca(2+)-dependent membrane-targeting module.
    The PH domain mediates interaction with the surface membrane by binding to PIP2.
  • Cellular localization
    Membrane. Cytoplasm. Cleavage furrow. Localizes at the cleavage furrow during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase delta-3 antibody
    • Phosphoinositide phospholipase C-delta-3 antibody
    • Phospholipase C-delta-3 antibody
    • PLC-delta-3 antibody
    • PLCD3 antibody
    • PLCD3_HUMAN antibody
    see all

Images

  • ab121859, at 1/200 dilution, staining PLC-delta-3 in hepatocytes of paraffin-embedded Human liver tissue by Immunohistochemistry.
  • All lanes : Anti-PLC-delta-3 antibody (ab121859) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

This product has been referenced in:
  • Yang K  et al. Effect of PLCe gene silencing on inhibiting the cancerous transformation of ulcerative colitis. Exp Ther Med 12:422-426 (2016). WB ; Mouse . Read more (PubMed: 27347072) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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