Overview

  • Product name
  • Description
    Mouse polyclonal to PLCD4
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Macaque monkey, Orangutan
  • Immunogen

    Full length protein corresponding to amino acids 1-762 of Human PLCD4. (NP_116115).

  • Positive control
    • PLCD4 transfected 293T cell lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 88 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.
  • Tissue specificity
    Highly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 3 EF-hand domains.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Domain
    The PDZ-binding motif mediates the interaction with GRIP1.
    The C2 domain mediates pre-localization to the membrane prior to Ca(2+) import and non-selective Ca(2+)-mediated targeting to various cellular membranes.
    The PH domain is not a critical determinant of the membrane localization.
  • Cellular localization
    Membrane. Nucleus. Cytoplasm. Endoplasmic reticulum. Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase delta 4 antibody
    • 1-phosphatidylinositol 4 antibody
    • 5-bisphosphate phosphodiesterase delta-4 antibody
    • hPLCD4 antibody
    • MGC12837 antibody
    • Phosphoinositide phospholipase C delta 4 antibody
    • Phosphoinositide phospholipase C-delta-4 antibody
    • Phospholipase C delta 4 antibody
    • Phospholipase C-delta-4 antibody
    • PLC delta 4 antibody
    • PLC delta4 antibody
    • PLC-delta-4 antibody
    • PLCD4 antibody
    • PLCD4_HUMAN antibody
    see all

Images

  • All lanes : Anti-PLCD4 antibody (ab169169) at 1/500 dilution

    Lane 1 : PLCD4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat anti-mouse HRP at 1/2500 dilution

    Predicted band size: 88 kDa

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PLCD4 with ab169169 at 10μg/ml.

References

ab169169 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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