Overview

  • Product nameAnti-PLCG 2 antibody
    See all PLCG 2 primary antibodies
  • Description
    Mouse polyclonal to PLCG 2
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PLCG 2 (AAH07565.1)

  • Positive control
    • Human liver tissue lysate, K-562 cell lysate, PLCG2 transfected 293T cell lysate and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 23555801
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 148 kDa.

Target

  • FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase gamma-2 antibody
    • EC 3.1.4.11 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-2 antibody
    • Phospholipase C gamma 2 antibody
    • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
    • Phospholipase C-gamma-2 antibody
    • Phospholipase C-IV antibody
    • PLC 2 antibody
    • PLC gamma 2 antibody
    • PLC IV antibody
    • PLC-gamma-2 antibody
    • PLC-IV antibody
    • Plcg2 antibody
    • PLCG2_HUMAN antibody
    see all

Anti-PLCG 2 antibody images

  • Anti-PLCG 2 antibody (ab89625) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 148 kDa
    Observed band size : 148 kDa
  • Anti-PLCG 2 antibody (ab89625) at 1 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 148 kDa
    Observed band size : 148 kDa
  • All lanes : Anti-PLCG 2 antibody (ab89625) at 1 µg/ml

    Lane 1 : PLCG 2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 148 kDa

References for Anti-PLCG 2 antibody (ab89625)

This product has been referenced in:
  • Bernal-Quirós M  et al. BANK1 and BLK act through phospholipase C gamma 2 in B-cell signaling. PLoS One 8:e59842 (2013). IP ; Human . Read more (PubMed: 23555801) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 20 µg
Specification HeLa Cells
Gel Running Conditions Non-reduced Denaturing (7.5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted May 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"