The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Ready to use for 30 minutes at room temperature. Requires boiling of tissue sections in 10mM citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.