PLCG 2 overexpression 293T lysate (whole cell) (ab94166)

Overview

  • Product namePLCG 2 overexpression 293T lysate (whole cell)
  • Description
    PLCG 2 overexpression lysate
  • General notesab94166 is a 293T cell transfected lysate in which Human PLCG 2 has been transiently over-expressed using a pCMV-PLCG 2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. Similarity: Contains 1 C2 domain. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. Contains 2 SH2 domains. Contains 1 SH3 domain. PTM: Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PLCG 2 overexpression 293T lysate (whole cell) images

  • ab94166 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PLCG 2 antibody (ab89625) at 1/500 dilution

    Lane 1 : PLCG 2 overexpression 293T lysate (whole cell) (ab94166)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for PLCG 2 overexpression 293T lysate (whole cell) (ab94166)

ab94166 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"