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Rabbit monoclonal [EP1405Y] to PLCG 2 (phospho Y1197)
ab75970 detects PLCG 2 phosphorylated on tyrosine 1197.
ICC more details Unsuitable for:
Flow Cyt or IHC-P
A phospho specific peptide corresponding to residues surrounding tyrosine 1197 of human PLCG 2.
Raji cell lysates treated with pervanadate.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
1/100 - 1/250.
Is unsuitable for Flow Cyt or IHC-P.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Contains 1 C2 domain.
Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
Information by UniProt
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
5-bisphosphate phosphodiesterase gamma-2 antibody
Western blot - Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970)
All lanes :
Anti-PLCG 2 (phospho Y1197) antibody [EP1405Y] (ab75970) at 1/20000 dilution
Lane 1 :
Raji cell lysates un-treated
Lane 2 :
Raji cell lysates treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size:
Observed band size:
150 kDa (
why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"