Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] (ab52610)

Overview

  • Product nameAnti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y]
    See all PLCG 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217 + Y1217)
  • Specificityab52610 only detects PLCG2 phosphorylated on Tyrosine 1217.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PLCG 2 aa 1200 to the C-terminus (phospho Y1217).

  • Positive control
    • Raji cell lysate; human lymph node tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IP 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
    • Sequence similaritiesContains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] images

    • All lanes : Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution

      Lane 1 : Raji cell lysate with no pervanadate
      Lane 2 : Raji cell lysate with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 150 kDa
    • ab52610 staining PLCG 2 (phospho Y1217) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized; antigen retrieval was by heat mediation in Tris EDTA buffer, pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human lymph node tissue using ab52610 at a dilution of 1/50.

    References for Anti-PLCG 2 (phospho Y1217 + Y1217) antibody [EP1404Y] (ab52610)

    ab52610 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
    Sample Human Tissue sections (Stomach)
    Specification Stomach
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted Mar 30 2015

    Thank you for your enquiry.

    Below please find the protocol used to test this antibody in IHC-P. Please note that citrate buffer pH 6 was used as the retreival buffer.

    I hope this is helpful. Please contact me again if you have any f...

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    Thank you for your enquiry regarding ab52610.

    The phosphorylation of PLCG 2 at Tyrosine 1217 needs to be induced to be able to detect this specific modification. As the WB image on the datasheet indicates Raji cell lysate without stimulation ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"