Anti-PLCG 2 (phospho Y753) antibody (ab75659)


  • Product name
    Anti-PLCG 2 (phospho Y753) antibody
    See all PLCG 2 primary antibodies
  • Description
    Rabbit polyclonal to PLCG 2 (phospho Y753)
  • Specificity
    ab75659 detects endogenous levels of PLCG 2 only when phosphorylated at tyrosine 753.
  • Tested applications
    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human PLCG 2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).

  • Positive control
    • Extract from A431 cells treated with EGF (200ng/ml, 5min).



Our Abpromise guarantee covers the use of ab75659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).


  • Function
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Post-translational
    Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase gamma-2 antibody
    • EC antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-2 antibody
    • Phospholipase C gamma 2 antibody
    • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
    • Phospholipase C-gamma-2 antibody
    • Phospholipase C-IV antibody
    • PLC 2 antibody
    • PLC gamma 2 antibody
    • PLC IV antibody
    • PLC-gamma-2 antibody
    • PLC-IV antibody
    • Plcg2 antibody
    • PLCG2_HUMAN antibody
    see all

Anti-PLCG 2 (phospho Y753) antibody images

  • PLCG 2 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to PLCG 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75659.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 148kDa; PLCG 2

  • All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution

    Lane 1 : A431 cell extracts, untreated
    Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)

    Predicted band size : 148 kDa
    Observed band size : 148 kDa
  • ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.

References for Anti-PLCG 2 (phospho Y753) antibody (ab75659)

ab75659 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75659.
Please use the links above to contact us or submit feedback about this product.


Sign up