The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
1/50 - 1/100.
Application notesIs unsuitable for Flow Cyt.
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Post-translational modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
References for Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)
This product has been referenced in:
Mohammad DK et al. Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3?, regulates shuttling, and attenuates both tonic and induced signaling in B cells. Mol Cell Biol33:3214-26 (2013).
Read more (PubMed: 23754751) »