Recombinant
RabMAb

Anti-PLCL1 antibody [EPR11213] (ab157200)

Overview

  • Product name
    Anti-PLCL1 antibody [EPR11213]
    See all PLCL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11213] to PLCL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PLCL1.

  • Positive control
    • Jurkat cell lysate; fetal brain, fetal kidney and fetal lung cell lysates; Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11213
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab157200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 123 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Involved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as a inhibitor of PPP1C.
    • Tissue specificity
      Expressed in a variety of fetal and adult organs including brain, lung and kidney. Its expression was greatly reduced in small and non-small cell lung carcinoma. Isoform 1 is predominantly expressed in brain.
    • Sequence similarities
      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • Post-translational
      modifications
      Phosphorylated by the catalytic subunit of PKA. Phosphorylation of Thr-93 resulted in dissociation of PPP1C from PRIP1.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Inactive phospholipase C-like protein 1 antibody
      • Inactive protein 1 antibody
      • Phospholipase C deleted in lung carcinoma antibody
      • Phospholipase C epsilon antibody
      • Phospholipase C like 1 antibody
      • Phospholipase C related but catalytically inactive protein antibody
      • Phospholipase C related, but catalytically inactive protein antibody
      • Phospholipase C-deleted in lung carcinoma antibody
      • Phospholipase C-related but catalytically inactive protein antibody
      • Phospholipase C-related catalytically antibody
      • PLC-L1 antibody
      • PLCE antibody
      • PLCL antibody
      • PLCL1 antibody
      • PLCL1_HUMAN antibody
      • PLDL1 antibody
      • PRIP antibody
      see all

    Images

    • Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing PLCL1, using ab157200 at 1/10 dilution for IP.
    • All lanes : Anti-PLCL1 antibody [EPR11213] (ab157200) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal lung lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 123 kDa

    • Immunofluorescence analysis of Jurkat cells, labeling PLCL1 using ab157200 at a 1/100 dilution.
    • Flow cytometry analysis of permeabilized Jurkat cells labeling PLCL1 (red), using ab157200 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab157200 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.01% triton
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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