Anti-PLCL2 antibody - C-terminal (ab170725)

Overview

  • Product name
    Anti-PLCL2 antibody - C-terminal
    See all PLCL2 primary antibodies
  • Description
    Rabbit polyclonal to PLCL2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PLCL2 aa 1100-1127 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UPR0

  • Positive control
    • NCI-H460 cells and lysate; Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170725 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 126 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function
    May play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Cellular localization
    Cytoplasm. Predominantly localized to perinuclear areas in both myoblast and myotube C2C12 cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ13484 antibody
    • Inactive phospholipase C like protein 2 antibody
    • Inactive phospholipase C-like protein 2 antibody
    • KIAA1092 antibody
    • Phospholipase C epsilon 2 antibody
    • Phospholipase C L2 antibody
    • Phospholipase C like 2 antibody
    • Phospholipase C-epsilon-2 antibody
    • Phospholipase C-L2 antibody
    • PLC epsilon 2 antibody
    • PLC L(2) antibody
    • PLC L2 antibody
    • PLC-epsilon-2 antibody
    • PLC-L(2) antibody
    • PLC-L2 antibody
    • PLCE 2 antibody
    • PLCE2 antibody
    • PLCL 2 antibody
    • PLCL2 antibody
    • PLCL2_HUMAN antibody
    see all

Images

  • Anti-PLCL2 antibody - C-terminal (ab170725) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 126 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling PLCL2  with ab170725 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Confocal imunofluorescent  analysis of NCI-H460 cells labeling PLCL2  with ab170725 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Flow cytometric analysis of NCI-H460 cells labeling PLCL2 with ab170725 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab170725 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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