Synthetic peptide within Human PLCL2 aa 1100-1127 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9UPR0
Our Abpromise guarantee covers the use of ab170725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 126 kDa.|
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling PLCL2 with ab170725 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal imunofluorescent analysis of NCI-H460 cells labeling PLCL2 with ab170725 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells labeling PLCL2 with ab170725 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
ab170725 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"