Overview

  • Product name
  • Description
    Rabbit polyclonal to PLCZ1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PLCZ1 (internal sequence).
    Database link: Q86YW0

  • Positive control
    • Human uterus endometrium tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab181816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
  • Tissue specificity
    Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • Domain
    The EF-hand and C2 domains are essential for triggering Ca(2+) oscillating activity and the regulation of PLCZ1 enzyme activity.
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.
  • Cellular localization
    Nucleus. Cytoplasm > perinuclear region. Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase zeta-1 antibody
    • MGC149685 antibody
    • NYD SP27 antibody
    • Phosphoinositide phospholipase C zeta 1 antibody
    • Phosphoinositide phospholipase C-zeta-1 antibody
    • Phospholipase C zeta 1 antibody
    • Phospholipase C-zeta-1 antibody
    • PI phospholipase C zeta 1 antibody
    • PLC zeta 1 antibody
    • PLC-zeta-1 antibody
    • PLCZ1 antibody
    • PLCZ1_HUMAN antibody
    • PLCzeta antibody
    • Testis development protein NYD SP27 antibody
    • Testis development related NYD SP27 antibody
    • Testis-development protein NYD-SP27 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus endometrium tissue labeling PLCZ1 with ab181816 at 10 µg/ml.

References

ab181816 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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