Overview

  • Product nameAnti-PLD3 antibody
    See all PLD3 primary antibodies
  • Description
    Rabbit polyclonal to PLD3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region of Human PLD3

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

Target

  • Tissue specificityWidely expressed. Expressed at higher level in brain. Expressed at low level in corpus callosum, suggesting that it is highly expressed in neurons.
  • Sequence similaritiesBelongs to the phospholipase D family.
    Contains 2 PLD phosphodiesterase domains.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choline phosphatase 3 antibody
    • HindIII K4L homolog antibody
    • HU K4 antibody
    • Hu-K4 antibody
    • HUK4 antibody
    • Phosphatidylcholine hydrolyzing phospholipase D3 antibody
    • Phosphatidylcholine-hydrolyzing phospholipase D3 antibody
    • Phospholipase D family, member 3 antibody
    • Phospholipase D3 antibody
    • PLD 3 antibody
    • PLD3 antibody
    • PLD3_HUMAN antibody
    see all

Anti-PLD3 antibody images



  • Predicted band size : 55 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling PLD3 with ab76433 at 1.0µg/ml.
  • Anti-PLD3 antibody (ab76433) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Gel concentration 12%

References for Anti-PLD3 antibody (ab76433)

ab76433 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification total larvae, 4 days post fertilization
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"