Anti-PLD6 antibody (ab170183)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-PLD6 antibody
See all PLD6 primary antibodies -
DescriptionRabbit polyclonal to PLD6
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Cow, Dog, Human
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Immunogen
Synthetic peptide within Human PLD6 aa 132-162 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8N2A8 -
Positive control
- Mouse testis lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab170183 is purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 28 kDa. |
Target
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FunctionCardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons.
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Tissue specificityWidely expressed. Expression is not limites to testis and ovary.
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Sequence similaritiesBelongs to the phospholipase D family. MitoPLD/Zucchini subfamily.
Contains 1 PLD phosphodiesterase domain. -
DomainIn contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site (PubMed:17028579).
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Cellular localizationMitochondrion outer membrane.
- Information by UniProt
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Database links
- Entrez Gene: 526651 Cow
- Entrez Gene: 489545 Dog
- Entrez Gene: 201164 Human
- Entrez Gene: 194908 Mouse
- Omim: 614960 Human
- SwissProt: E1BE10 Cow
- SwissProt: E2RD63 Dog
- SwissProt: Q8N2A8 Human
see all -
Alternative names
- Choline phosphatase 6 antibody
- Mitochondrial cardiolipin hydrolase antibody
- Mitochondrial phospholipase antibody
see all
Images
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Western blot - Anti-PLD6 antibody (ab170183)Anti-PLD6 antibody (ab170183) at 1/100 dilution + mouse testis lysate at 35 µg
Predicted band size: 28 kDa
Protocols
References
This product has been referenced in:
- Zhou Q et al. Establishment of a proteome profile and identification of molecular markers for mouse spermatogonial stem cells. J Cell Mol Med 19:521-34 (2015). IF . Read more (PubMed: 25352495) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"