Overview

  • Product name
  • Description
    Goat polyclonal to Pleckstrin
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    EWIKAIQMASRTGK

    with a Cysteine residue linker, corresponding to C terminal amino acids 337-350 of Human Pleckstrin (NP_002655.2).

  • Positive control
    • Human blood vessel (platelets) and spleen tissue; Human PBMC lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 40 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human vessel (platelets) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-Pleckstrin antibody (ab115514) at 0.05 µg/ml + Human PBMC lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 40 kDa

References

ab115514 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (IC21)
Gel Running Conditions
Reduced Denaturing (4-20%, 100V)
Loading amount
10 µg
Treatment
shRNA
Specification
IC21
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Spencer Goodman

Verified customer

Submitted May 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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