The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 500 kDa (predicted molecular weight: 531 kDa).
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml.
FunctionInterlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
Tissue specificityWidely expressed with highest levels in muscle, heart, placenta and spinal cord.
Involvement in diseaseEpidermolysis bullosa simplex with pyloric atresia Epidermolysis bullosa simplex, with muscular dystrophy Epidermolysis bullosa simplex, Ogna type Limb-girdle muscular dystrophy 2Q
Sequence similaritiesBelongs to the plakin or cytolinker family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 33 plectin repeats. Contains 4 spectrin repeats.
DomainThe N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
Post-translational modificationsPhosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
ICC/IF image of ab83497 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83497, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Plectin antibody (ab83497)
This product has been referenced in:
Rikardsen OG et al. Plectin as a prognostic marker in non-metastatic oral squamous cell carcinoma. BMC Oral Health15:98 (2015).
Read more (PubMed: 26306491) »
Burch TC et al. Variable metastatic potentials correlate with differential plectin and vimentin expression in syngeneic androgen independent prostate cancer cells. PLoS One8:e65005 (2013).
Read more (PubMed: 23717685) »