Anti-Plectin antibody [E398P] (ab32528)


  • Product name
    Anti-Plectin antibody [E398P]
    See all Plectin primary antibodies
  • Description
    Rabbit monoclonal [E398P] to Plectin
  • Specificity
    ab32528 recognises plectin, a 500-kDa intermediate filament binding protein.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A recombinant fragment comprising the C-terminal 220 amino acids of plectin.

  • Positive control
    • Jurkat cell lysate, human skeletal muscle
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab32528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 500 kDa (predicted molecular weight: 531 kDa).
IHC-P 1/250.
IP 1/40.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
    • Tissue specificity
      Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
    • Involvement in disease
      Epidermolysis bullosa simplex with pyloric atresia
      Epidermolysis bullosa simplex, with muscular dystrophy
      Epidermolysis bullosa simplex, Ogna type
      Limb-girdle muscular dystrophy 2Q
    • Sequence similarities
      Belongs to the plakin or cytolinker family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 33 plectin repeats.
      Contains 4 spectrin repeats.
    • Domain
      The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
    • Post-translational
      Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • EBS1 antibody
      • EBSO antibody
      • HD1 antibody
      • Hemidesmosomal protein 1 antibody
      • PCN antibody
      • pleC antibody
      • PLEC_HUMAN antibody
      • PLEC1 antibody
      • PLEC1b antibody
      • Plectin 1 antibody
      • Plectin 1 intermediate filament binding protein 500kDa antibody
      • Plectin 6 antibody
      • Plectin antibody
      • Plectin-1 antibody
      • PLTN antibody
      see all


    • Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250
    • Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI


    This product has been referenced in:
    • Croner RS  et al. Identification of Predictive Markers for Response to Neoadjuvant Chemoradiation in Rectal Carcinomas by Proteomic Isotope Coded Protein Label (ICPL) Analysis. Int J Mol Sci 17:209 (2016). IHC . Read more (PubMed: 26861291) »
    • Ketema M  et al. The rod domain is not essential for the function of plectin in maintaining tissue integrity. Mol Biol Cell 26:2402-17 (2015). Read more (PubMed: 25971800) »

    See all 17 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit monoclonal [E398P] to Plectin (ab32528) is yet to be tested for use by immunocytochemistry and therefore we cannot guarantee it for thi...

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