Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab55636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionHeparin binding mitogenic protein. Has neurite extension activity.
  • Tissue specificityOsteoblast and brain.
  • Sequence similaritiesBelongs to the pleiotrophin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HARP antibody
    • HB-GAM antibody
    • HBBM antibody
    • HBGAM antibody
    • HBGF-8 antibody
    • HBGF8 antibody
    • HBNF antibody
    • HBNF-1 antibody
    • HBNF1 antibody
    • heparin affin regulatory protein antibody
    • Heparin binding growth associated molecule antibody
    • Heparin binding growth factor 8 antibody
    • Heparin binding neurite outgrowth promoting factor 1 antibody
    • Heparin-binding brain mitogen antibody
    • Heparin-binding growth factor 8 antibody
    • Heparin-binding growth-associated molecule antibody
    • heparin-binding neurite outgrowth promoting factor antibody
    • Heparin-binding neurite outgrowth-promoting factor 1 antibody
    • NEGF1 antibody
    • Neurite growth promoting factor 1 antibody
    • Neurite outgrowth-promoting factor, heparin-binding antibody
    • OSF-1 antibody
    • OSF1 antibody
    • Osteoblast-specific factor 1 antibody
    • pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) antibody
    • Pleiotrophin antibody
    • PTN antibody
    • PTN_HUMAN antibody
    see all

Anti-Pleiotrophin antibody images

  • Pleiotrophin antibody (ab55636) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.


  • Predicted band size : 19 kDa
  • ICC/IF image of ab55636 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55636, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Pleiotrophin antibody (ab55636)

ab55636 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"