Anti-Pleiotrophin antibody - C-terminal (ab223674)

Overview

  • Product name
    Anti-Pleiotrophin antibody - C-terminal
    See all Pleiotrophin primary antibodies
  • Description
    Goat polyclonal to Pleiotrophin - C-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Pleiotrophin aa 158-168 (C terminal) (Cysteine residue). NP_002816.1.
    Sequence:

    C-KEGKKQEKMLD


    Database link: P21246

  • Positive control
    • WB: Mouse fetal brain lysate. IHC-P: Human prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab223674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18.9 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Heparin binding mitogenic protein. Has neurite extension activity.
  • Tissue specificity
    Osteoblast and brain.
  • Sequence similarities
    Belongs to the pleiotrophin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HARP antibody
    • HB-GAM antibody
    • HBBM antibody
    • HBGAM antibody
    • HBGF-8 antibody
    • HBGF8 antibody
    • HBNF antibody
    • HBNF-1 antibody
    • HBNF1 antibody
    • heparin affin regulatory protein antibody
    • Heparin binding growth associated molecule antibody
    • Heparin binding growth factor 8 antibody
    • Heparin binding neurite outgrowth promoting factor 1 antibody
    • Heparin-binding brain mitogen antibody
    • Heparin-binding growth factor 8 antibody
    • Heparin-binding growth-associated molecule antibody
    • heparin-binding neurite outgrowth promoting factor antibody
    • Heparin-binding neurite outgrowth-promoting factor 1 antibody
    • NEGF1 antibody
    • Neurite growth promoting factor 1 antibody
    • Neurite outgrowth-promoting factor, heparin-binding antibody
    • OSF-1 antibody
    • OSF1 antibody
    • Osteoblast-specific factor 1 antibody
    • pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) antibody
    • Pleiotrophin antibody
    • PTN antibody
    • PTN_HUMAN antibody
    see all

Images

  • Paraffin-embedded human prostate tissue stained for Pleiotrophin using ab223674 at 3.8 µg/ml in immunohistochemical analysis, followed by AP-staining.

  • Anti-Pleiotrophin antibody - C-terminal (ab223674) at 0.1 µg/ml + mouse fetal brain lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 18.9 kDa
    Observed band size: 18 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab223674 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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