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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Retrieval buffer pH 6.
- FunctionMay help orchestrate cytoskeletal arrangement. Contribute to lamellipodia formation.
- Sequence similaritiesContains 1 DEP domain.
Contains 2 PH domains.
- Cellular localizationCell projection > lamellipodium membrane. Cytoplasm > cytoskeleton.
Information by UniProt
- Pleckstrin 2 (mouse) homolog antibody
- Pleckstrin 2 antibody
- Pleckstrin-2 antibody
Anti-PLEK2 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-PLEK2 antibody (ab121131)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab121131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-PLEK2 antibody (ab121131)
All lanes : Anti-PLEK2 antibody (ab121131) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEK2 antibody (ab121131)
ab121131 at 1/50 dilution staining PLEK2 in Paraffin-emebedded Human liver tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in hepatocytes.
References for Anti-PLEK2 antibody (ab121131)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"