Overview

  • Product nameAnti-PLEK2 antibody
    See all PLEK2 primary antibodies
  • Description
    Rabbit polyclonal to PLEK2
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 237-346 of Human PLEK2 (Q9NYT0).

  • Positive control
    • Human liver tissue and lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Retrieval buffer pH 6.

Target

Anti-PLEK2 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab121131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-PLEK2 antibody (ab121131) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121131 at 1/50 dilution staining PLEK2 in Paraffin-emebedded Human liver tissue by Immunohistochemistry. Note: strong cytoplasmic positivity in hepatocytes.

References for Anti-PLEK2 antibody (ab121131)

ab121131 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"