Overview

  • Product name
    Anti-PLEKHA2 antibody
  • Description
    Rabbit polyclonal to PLEKHA2
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 52-189 of Human PLEKHA2.

  • Positive control
    • Human Pancreas tissue; RT-4 and U-251 MG cell lysate; Human Tonsil lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Binds specifically to phosphatidylinositol-3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
  • Tissue specificity
    Highly expressed in heart, kidney, spleen and peripheral blood leukocytes. Detected at lower levels in brain, skeletal muscle, colon, thymus, liver, small intestine, placenta and lung.
  • Sequence similarities
    Contains 2 PH domains.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.
  • Information by UniProt
  • Database links
  • Alternative names
    • PH domain-containing family A member 2 antibody
    • PKHA2_HUMAN antibody
    • Pleckstrin homology domain-containing family A member 2 antibody
    • PLEKHA2 antibody
    • Tandem PH domain-containing protein 2 antibody
    • TAPP-2 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121218 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121218, at 1/200 dilution, staining PLEKHA2 in paraffin-embedded Human pancreas tissue, by Immunohistochemistry.
  • All lanes : Anti-PLEKHA2 antibody (ab121218) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

References

ab121218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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