Anti-PLEKHA3 antibody [2D1] (ab117849)

Overview

  • Product nameAnti-PLEKHA3 antibody [2D1]
    See all PLEKHA3 primary antibodies
  • Description
    Mouse monoclonal [2D1] to PLEKHA3
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length recombinant Human PLEKHA3 (NP_061964) produced in HEK293T cells.

  • Positive control
    • HEK293T transfected lysate HeLa or Jurkat cells HEK293T transfected cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from mouse ascite fluid by affinity chromatography
  • ClonalityMonoclonal
  • Clone number2D1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 34 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionBinds specifically to phosphatidylinositol-4-phosphate (PtdIns4P), but not to other phosphoinositides.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 PH domain.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FAPP 1 antibody
    • FAPP-1 antibody
    • FAPP1 antibody
    • FLJ20067 antibody
    • PH domain-containing family A member 3 antibody
    • Phosphatidylinositol-four-phosphate adapter protein 1 antibody
    • Phosphoinositol 4 phosphate adaptor protein 1 antibody
    • Phosphoinositol 4-phosphate adapter protein 1 antibody
    • PKHA3_HUMAN antibody
    • Pleckstrin homology domain containing family A (phosphoinositide binding specific) member 3 antibody
    • Pleckstrin homology domain containing family A member 3 antibody
    • Pleckstrin homology domain-containing family A member 3 antibody
    • PLEKHA 3 antibody
    • Plekha3 antibody
    see all

Anti-PLEKHA3 antibody [2D1] images

  • All lanes : Anti-PLEKHA3 antibody [2D1] (ab117849) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY PLEKHA3 cDNA for 48 hrs

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 34 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200386)
  • Immunofluorescent staining of HeLa cells using ab117849 at a 1/100 dilution.
  • Flow cytometry image showing ab117849 at a 1/100 dilution, staining HEK293T cells transfected with PLEKHA3 (Red), and an empty vector control plasmid (Blue).
  • Flow cytometry image staining HeLa cells, using ab117849 at a 1/100 dilution (Red), and a nonspecific negative control antibody (Blue).
  • Flow cytometry image staining Jurkat cells, using ab117849 at a 1/100 dilution (Red), and a nonspecific negative control antibody (Blue).

References for Anti-PLEKHA3 antibody [2D1] (ab117849)

ab117849 has not yet been referenced specifically in any publications.

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Below please find the IF protocol recommended with this antibody:

Fixation permeabilization

Coat the coverslips with 0.1mg/ml poly-L-lysine solution at room temperature for 2hrs, dry, and then w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"