Overview

  • Product nameAnti-PLEKHA4 antibody
    See all PLEKHA4 primary antibodies
  • Description
    Rabbit polyclonal to PLEKHA4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within residues: TEPDSPSPVL QGEESSERES LPESLELSSP RSPETDWGRP PGGDKDLASP , corresponding to internal sequence amino acids 504-553 of Human PLEKHA4 (NP_065955)

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionBinds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.
  • Tissue specificityHighly expressed in melanoma. Detected at low levels in heart, skeletal muscle, kidney, liver and small intestine.
  • Sequence similaritiesContains 1 PH domain.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410005C22Rik antibody
    • C88169 antibody
    • MGC72599 antibody
    • PEPP-1 antibody
    • PEPP1 antibody
    • PH domain-containing family A member 4 antibody
    • Phosphoinositol 3 phosphate binding protein 1 antibody
    • Phosphoinositol 3-phosphate-binding protein 1 antibody
    • PKHA4_HUMAN antibody
    • Pleckstrin homology domain containing family A member 4 antibody
    • Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 antibody
    • Pleckstrin homology domain-containing family A member 4 antibody
    • PLEKHA4 antibody
    see all

Anti-PLEKHA4 antibody images

  • Anti-PLEKHA4 antibody (ab84727) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
    Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PLEKHA4 antibody (ab84727)

ab84727 has not yet been referenced specifically in any publications.

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