Overview

  • Product nameAnti-PLEKHH2 antibody
  • Description
    Rabbit polyclonal to PLEKHH2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1045-1094 (LALCVGLFLP HHPFLWLLRL HLKRNADSRT EFGKYAIYCQ RCVERTQQNG) of Human PLEKHH2 (NP_742066).

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 168 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-PLEKHH2 antibody images

  • Anti-PLEKHH2 antibody (ab81300) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 168 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab81300 at 10 µg/ml staining PLEKHH2 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab81300 at 10 µg/ml staining PLEKHH2 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-PLEKHH2 antibody (ab81300)

ab81300 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab81300.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"