Overview

  • Product nameAnti-PLEKHO1 antibody
    See all PLEKHO1 primary antibodies
  • Description
    Mouse polyclonal to PLEKHO1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-409 of Human PLEKHO1 (NP_057358.2)

  • Positive control
    • Hela cell lysate, transfected 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.

Target

  • FunctionPlays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
  • Tissue specificityAbundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cancer cell lines such as MOLT-4, HEK293 and Jurkat cells.
  • Sequence similaritiesContains 1 PH domain.
  • Post-translational
    modifications
    C-terminal fragments could be released during apoptosis via caspase-3-dependent cleavage.
  • Cellular localizationCell membrane. Nucleus. Cytoplasm. Predominantly localized to the plasma membrane. In C2C12 cells, with the absence of growth factor, it is found in the nucleus. It rapidly translocates to the plasma membrane after insulin stimulation. In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3. However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-Jun-binding protein antibody
    • Casein kinase 2-interacting protein 1 antibody
    • CK2 interacting protein 1 antibody
    • CK2 interacting protein 1 HQ0024c protein antibody
    • CK2-interacting protein 1 antibody
    • CKIP 1 antibody
    • CKIP-1 antibody
    • CKIP1 antibody
    • JBP antibody
    • OC120 antibody
    • Osteoclast maturation associated gene 120 protein antibody
    • Osteoclast maturation-associated gene 120 protein antibody
    • PH domain-containing family O member 1 antibody
    • PKHO1_HUMAN antibody
    • Pleckstrin homology domain containing family O member 1 antibody
    • Pleckstrin homology domain-containing family O member 1 antibody
    • PLEKHO1 antibody
    see all

Anti-PLEKHO1 antibody images

  • Anti-PLEKHO1 antibody (ab89110) at 1 µg/ml + HeLa lysate at 50 µg

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PLEKHO1 antibody (ab89110) at 1 µg/ml

    Lane 1 : PLEKHO1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of purified ab89110 to PLEKHO1 on HeLa cell. [antibody concentration 10 ug/ml]

References for Anti-PLEKHO1 antibody (ab89110)

ab89110 has not yet been referenced specifically in any publications.

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