The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 195 kDa.
1/50 - 1/200.
1/200. (see Abreview).
Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
Tissue specificity: Detected in fetal brain, lung, liver and kidney.
Similarity: Belongs to the plexin family.
Contains 4 IPT/TIG domains.
Contains 1 Sema domain.
Western blots showing Cos-7 cells transfected with mouse myc-tagged Plexin-A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from myc-tagged Plexin-A1 transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (ab23391; lane 5). These blots were probed with either the affinity purified anti-Plexin-A1 (ab23391; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Delloye-Bourgeois C et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci18:36-45 (2015).
Read more (PubMed: 25485759) »
Hernandez-Enriquez B et al. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing. Genes Dev29:2617-32 (2015).
Read more (PubMed: 26680304) »