Anti-Plexin A1 antibody [EPR3449] (ab92346)

Overview

  • Product nameAnti-Plexin A1 antibody [EPR3449]
    See all Plexin A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3449] to Plexin A1
  • Specificityab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Plexin A1.

  • Positive control
    • KS62, HUVEC and HepG2 cell lysates; fetal brain tissue lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab92346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
Flow Cyt
  • Application notesFlow Cyt: 1/50.
    ICC: 1/100.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 211 kDa.

    Is unsuitable for IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Plexin A1 antibody [EPR3449] images

    • All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Fetal brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 211 kDa

    References for Anti-Plexin A1 antibody [EPR3449] (ab92346)

    ab92346 has not yet been referenced specifically in any publications.

    Product Wall

    Gracias por tu respuesta.

    Me temo que no puedo mandarte ambos anticuerpos, pero en caso de que el nuevo vial que te enviemos no te funcionara tampoco, éste también estaría cubierto por la garantía Abpromise, y por ...

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    Gracias por enviarnos los resultados.
    Si te interesa puedo enviarte otro vial de un lote diferente, o incluso un vial de otro anti-Plexin A1, el ab32960:
    http://www.abcam.com/Plexin-A1-antibody-ab32960.html
    A pesar de que la garantía ha ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Spleen)
    Loading amount 20 µg
    Specification Spleen
    Gel Running Conditions Reduced Denaturing (gel 7,5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 16 2012

    Thank you for contacting Abcam.   I have asked the lab to provide the protocol that they used to test ab92346 in flow cytometry is attached(also attached is the data from the lab). They also informed that ab92346 recognizes the extracellular domain of ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"