Recombinant
RabMAb

Anti-Plexin A1 antibody [EPR3449] (ab92346)

Overview

  • Product name
    Anti-Plexin A1 antibody [EPR3449]
    See all Plexin A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3449] to Plexin A1
  • Specificity
    ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Plexin A1.

  • Positive control
    • KS62, HUVEC and HepG2 cell lysates; fetal brain tissue lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
Flow Cyt
  • Application notes
    Flow Cyt: 1/50.
    ICC: 1/100.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 211 kDa.

    Is unsuitable for IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain.
    • Cellular localization
      Cell Membrane
    • Database links
    • Alternative names
      • NOV antibody
      • NOV/plexin A1 protein antibody
      • NOVP antibody
      • Plexin-A1 antibody
      • Plexin1 antibody
      • PlexinA1 antibody
      • PLXA1_HUMAN antibody
      • PLXN1 antibody
      • Plxna1 antibody
      • PLXNA1 protein antibody
      • Semaphorin receptor NOV antibody
      see all

    Images

    • All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Fetal brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 211 kDa

    References

    ab92346 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Spleen)
    Loading amount
    20 µg
    Specification
    Spleen
    Gel Running Conditions
    Reduced Denaturing (gel 7,5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 16 2012

    Ab92346 recognizes the extracellular domain of Plexin A1. Permeabilization is still required, even though the immunogen is extracellular.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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