Anti-Plexin A2 antibody (ab39357)
Key features and details
- Rabbit polyclonal to Plexin A2
- Suitable for: WB, IHC-P, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Plexin A2 antibody -
Description
Rabbit polyclonal to Plexin A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: HumanDoes not react with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse Plexin A2 aa 300-400.
(Peptide available asab39356) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa). |
IHC-P
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. |
Target
-
Function
Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. -
Tissue specificity
Detected in fetal brain. -
Sequence similarities
Belongs to the plexin family.
Contains 4 IPT/TIG domains.
Contains 1 Sema domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 5362 Human
- Entrez Gene: 18845 Mouse
- Omim: 601054 Human
- SwissProt: O75051 Human
- SwissProt: P70207 Mouse
- Unigene: 497626 Human
- Unigene: 2251 Mouse
-
Alternative names
- Plexin-A2 antibody
- PlexinA2 antibody
- PLXA2 antibody
see all
Images
-
Anti-Plexin A2 antibody (ab39357) at 2 µg/ml +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 212 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab39357 has been referenced in 6 publications.
- Szczurkowska J et al. Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development. Cell Rep 38:110483 (2022). PubMed: 35294878
- Bi-Lin KW et al. Critical role of the BAF chromatin remodeling complex during murine neural crest development. PLoS Genet 17:e1009446 (2021). PubMed: 33750945
- Smolkin T et al. Complexes of plexin-A4 and plexin-D1 convey semaphorin-3C signals to induce cytoskeletal collapse in the absence of neuropilins. J Cell Sci 131:N/A (2018). PubMed: 29661844
- Sabag AD et al. The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction. J Cell Sci 127:5240-52 (2014). PubMed: 25335892
- Li W et al. Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. Proc Natl Acad Sci U S A 110:1738-43 (2013). PubMed: 23319608
- Piaton G et al. Class 3 semaphorins influence oligodendrocyte precursor recruitment and remyelination in adult central nervous system. Brain 134:1156-67 (2011). PubMed: 21421691