Overview

  • Product name
  • Description
    Rabbit polyclonal to Plexin A2
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: HumanDoes not react with: Rat
  • Immunogen

    Synthetic peptide derived from within residues 300 - 400 of Mouse Plexin A2.

    (Peptide available as ab39356.)

  • Positive control
    • This antibody gave a positive signal in Mouse Brain (0 Day) Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab39357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).
IHC-FoFr 1/300.

Target

  • Function
    Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
  • Tissue specificity
    Detected in fetal brain.
  • Sequence similarities
    Belongs to the plexin family.
    Contains 4 IPT/TIG domains.
    Contains 1 Sema domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Plexin-A2 antibody
    • PlexinA2 antibody
    • PLXA2 antibody
    • PLXA2_HUMAN antibody
    • PLXNA2 antibody
    • Semaphorin receptor OCT antibody
    see all

Images

  • Anti-Plexin A2 antibody (ab39357) at 2 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary
    Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 212 kDa
    Observed band size : 230 kDa (why is the actual band size different from the predicted?)

    Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • IHC-FoFr image of Plexin A2 staining on mouse cortex (Lamina V) using ab39357 (1/300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

  • Immunofluorescence analysis of mouse neural tube cells, staining Plexin A2 (green) with ab39357.

    Cells were fixed before being blocked with 10% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Sabag AD  et al. The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction. J Cell Sci 127:5240-52 (2014). Read more (PubMed: 25335892) »
  • Li W  et al. Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. Proc Natl Acad Sci U S A 110:1738-43 (2013). Read more (PubMed: 23319608) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neural Tube)
Specification
Neural Tube
Fixative
IF-P
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Nov 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 08 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (embryo d15 spinal cord, kidney, adult brain)
Specification
embryo d15 spinal cord, kidney, adult brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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