Overview

  • Product nameAnti-Plexin A2 antibody
    See all Plexin A2 primary antibodies
  • Description
    Mouse monoclonal to Plexin A2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Recombinant Fragment
  • Immunogen

    Recombinant full length, Human Plexin A2 (AAH32125) with a GST tag (MW of the GST tag is 26 KDa).

  • Positive control
    • PC12, HepG2, Raw 264.7 and NIH/3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab88130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 237 kDa (predicted molecular weight: 211 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionCoreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
  • Tissue specificityDetected in fetal brain.
  • Sequence similaritiesBelongs to the plexin family.
    Contains 4 IPT/TIG domains.
    Contains 1 Sema domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Plexin-A2 antibody
    • PlexinA2 antibody
    • PLXA2 antibody
    • PLXA2_HUMAN antibody
    • PLXNA2 antibody
    • Semaphorin receptor OCT antibody
    see all

Anti-Plexin A2 antibody images

  • Anti-Plexin A2 antibody (ab88130) at 1 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 211 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • Anti-Plexin A2 antibody (ab88130) at 1 µg/ml + PC12 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 211 kDa
    Observed band size : 237 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Plexin A2 antibody (ab88130) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 211 kDa
    Observed band size : 237 kDa (why is the actual band size different from the predicted?)
  • Anti-Plexin A2 antibody (ab88130) at 1 µg/ml + Raw 264.7 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 211 kDa
    Observed band size : 237 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Plexin A2 antibody (ab88130) at 1 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 211 kDa
    Observed band size : 237 kDa (why is the actual band size different from the predicted?)

References for Anti-Plexin A2 antibody (ab88130)

ab88130 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - other (Mesenchymal stem cell)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Mesenchymal stem cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Apr 14 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lymphoblastoid, SH-SY5Y)
Loading amount 20 µg
Specification Lymphoblastoid, SH-SY5Y
Gel Running Conditions Reduced Denaturing (4-12% BIS TRIS)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"