The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 237 kDa (predicted molecular weight: 211 kDa).
Use at an assay dependent dilution.
FunctionCoreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
Tissue specificityDetected in fetal brain.
Sequence similaritiesBelongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain.