Overview

  • Product name
  • Description
    Rabbit polyclonal to Plexin A3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from within residues 1100 - 1200 of Mouse Plexin A3.

    (Peptide available as ab41563.)

  • Positive control
    • This antibody gave a positive signal in P0 mouse brain and mouse hippocampus tissue lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab41564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Plexin A3 is a coreceptor for the class 3 secreted semaphorins. This family of semaphorins binds to a complex composed of a neuropilin and a plexin. The plexin type modulates the affinity of the complex for specific semaphorins and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Plexin A3 is a putative receptor involved in the development of neural and epithelial tissues.
  • Cellular localization
    Membrane; single-pass type I membrane protein.
  • Database links
  • Alternative names
    • HSSEXGENE antibody
    • Plexin 4 antibody
    • PLXN4 antibody
    • PLXNA3 antibody
    • Semaphorin receptor SEX antibody
    • SEX antibody
    • Transmembrane protein sex antibody
    • XAP 6 antibody
    see all

Anti-Plexin A3 antibody images

  • All lanes : Anti-Plexin A3 antibody (ab41564) at 1 µg/ml

    Lane 1 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 207 kDa
    Observed band size : 207 kDa
    Additional bands at : 32 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Plexin A3 antibody (ab41564)

This product has been referenced in:
  • Demyanenko GP  et al. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci 34:11274-87 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 25143608) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cells)
Loading amount
22 µg
Specification
Cancer cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

Submitted Jul 23 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E 14 Mouse Embryo)
Specification
E 14 Mouse Embryo
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Manoj Kumar Valluru

Verified customer

Submitted Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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