Anti-Plexin B1 antibody (ab39717)

Overview

  • Product nameAnti-Plexin B1 antibody
    See all Plexin B1 primary antibodies
  • Description
    Rabbit polyclonal to Plexin B1
  • SpecificityPLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved.
  • Tested applicationsSuitable for: IHC-FoFr, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.

    (Peptide available as ab41062.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver Rat Liver Rat Pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab39717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).

Target

  • RelevancePlexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton.
  • Cellular localizationSecreted and single pass type I membrane protein.
  • Database links
  • Alternative names
    • KIAA0407 antibody
    • Plexin 5 antibody
    • Plexin B1 antibody
    • PLXN5 antibody
    • PLXNB1 antibody
    • Semaphorin receptor antibody
    • Semaphorin receptor SEP antibody
    • SEP antibody
    see all

Anti-Plexin B1 antibody images

  • All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 233 kDa
    Observed band size : 171 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 155 kDa. We are unsure as to the identity of these extra bands.
    We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1.
  • Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).

References for Anti-Plexin B1 antibody (ab39717)

ab39717 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Primary dissection of hippocampal neurons.)
Specification Primary dissection of hippocampal neurons.
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Michael Weible

Verified customer

Submitted Nov 08 2012



Expiration date: 2012-11-09


I am very pleased to hear you would like to accept our offer and test

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"