• Product name
  • Description
    Rabbit polyclonal to Plexin B1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide from near N terminal residues of Human Plexin B1 (NM_002673).

  • Positive control
    • Rat kidney tissue; fetal kidney lysate, rat brain lysate and rat testis lysate



Our Abpromise guarantee covers the use of ab90087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 232 kDa.Can be blocked with Plexin B1 peptide (ab196855).
IHC-P 1/50 - 1/200.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.


  • Relevance
    Plexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton.
  • Cellular localization
    Secreted and single pass type I membrane protein.
  • Database links
  • Alternative names
    • KIAA0407 antibody
    • Plexin 5 antibody
    • Plexin B1 antibody
    • PLXN5 antibody
    • PLXNB1 antibody
    • Semaphorin receptor antibody
    • Semaphorin receptor SEP antibody
    • SEP antibody
    see all

Anti-Plexin B1 antibody images

  • Plexin B1 was immunoprecipitated using 0.5mg Rat brain tissue extract, 5µg of Rabbit polyclonal to Plexin B1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Rat brain tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90087.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 232kDa; Plexin B1

  • All lanes : Anti-Plexin B1 antibody (ab90087) at 1/500 dilution

    Lane 1 : rat brain lysate
    Lane 2 : rat testis lysate
    Lane 3 : fetal kidney lysate

    Predicted band size : 232 kDa
  • Immunohistochemical staining of formalin-fixed paraffin-embedded rat kidney using ab90087 at 1/100.
  • ab90087 staining Plexin B1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in PBS + 2% Blocking Serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab90087 stained PC12 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90087, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Plexin B1 antibody (ab90087)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Mr. Manoj Kumar Valluru

Verified customer

Submitted Jun 04 2010


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