ab28762 at 1/300 dilution, staining Plexin D1 in mouse brain tissue sections by IHC-P (Formalin / PFA fixed prafaffin embedded sections). Tissue underwent formaldehyde fixation, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocked in 5% serum for 1 hour. The primary antibody was used at 1/300 dillution for 1 hour. A Biotin-conjugated rabbit polyclonal to goat IgG was used as secondary at 1/250 dillution.
Parikh T et al. Proteomic analyses of corneal tissue subjected to alkali exposure. Invest Ophthalmol Vis Sci52:1819-31 (2011).
Read more (PubMed: 20861482) »
Sakurai A et al. Semaphorin 3E initiates antiangiogenic signaling through plexin D1 by regulating Arf6 and R-Ras. Mol Cell Biol30:3086-98 (2010).
WB, ICC/IF, IP
Human, Mouse, African green monkey
Read more (PubMed: 20385769) »