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Rabbit polyclonal to Plexin D1
Predicted to work with:
Synthetic peptide corresponding to amino acids 1633-1694 (RKKLNTLAHY KIPEGASLAM SLIDKKDNTL GRVKDLDTEK YFHLVLPTDE LAEPKKSHRQ SH) of Human Plexin D1.
HepG2 whole cell lysates and HeLa cell lysate
This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 40% Glycerol, 1 x PBS, pH 7.0
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/1000. Predicted molecular weight: 212 kDa.
Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. Detected at very low levels in brain, colon, spleen, small intestine and peripheral blood leukocytes.
Belongs to the plexin family.
Contains 3 IPT/TIG domains. Contains 1 Sema domain.
Information by UniProt
PLEX D1 antibody
Western blot - Anti-Plexin D1 antibody (ab96313)
Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution + HepG2 whole cell lysates at 30 µg
Predicted band size: 212 kDa
Western blot - Plexin D1 antibody (ab96313)
Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 212 kDa 5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (ab96313)
ab96313 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96313 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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