Overview

  • Product name
  • Description
    Rabbit polyclonal to Plexin D1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to amino acids 1633-1694 (RKKLNTLAHY KIPEGASLAM SLIDKKDNTL GRVKDLDTEK YFHLVLPTDE LAEPKKSHRQ SH) of Human Plexin D1.

  • Positive control
    • HepG2 whole cell lysates and HeLa cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: PC12

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1 x PBS, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 212 kDa.

Target

Images

  • Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution + HepG2 whole cell lysates at 30 µg

    Predicted band size : 212 kDa
  • Anti-Plexin D1 antibody (ab96313) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 212 kDa
    5% SDS PAGE
  • ab96313 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96313 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Shang G  et al. Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex. Elife 6:N/A (2017). Read more (PubMed: 28537552) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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