The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 25 kDa.
Detects 25-50 ng/lane of recombinant human PlGF1 and PlGF2 under reducing and non-reducing conditions.
Use a concentration of 1 - 5 µg/ml. Allows the detection of 1.0- 2.5 ng/well of recombinant human PlGF1 and PlGF2.
FunctionGrowth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
Tissue specificityWhile the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
DomainIsoform PlGF-2 contains a basic insert which acts as a cell retention signal.
Cellular localizationSecreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.