Anti-PLGF antibody (ab196666)
Key features and details
- Rabbit polyclonal to PLGF
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PLGF antibody
See all PLGF primary antibodies -
Description
Rabbit polyclonal to PLGF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PLGF (N terminal).
Database link: P49763-3 -
Positive control
- BT474 cell extract; Mouse brain tissue extract; Rat testis, Human stomach cancer and Rat kidney tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS (without Mg2+and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 25 kDa. |
Target
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Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
Tissue specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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Database links
- Entrez Gene: 5228 Human
- Entrez Gene: 18654 Mouse
- Entrez Gene: 94203 Rat
- Omim: 601121 Human
- SwissProt: P49763 Human
- SwissProt: P49764 Mouse
- SwissProt: Q63434 Rat
- Unigene: 252820 Human
see all -
Alternative names
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
see all
Images
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All lanes : Anti-PLGF antibody (ab196666) at 1/500 dilution
Lane 1 : BT474 cell extract
Lane 2 : Mouse brain tissue extract
Predicted band size: 25 kDa -
Immunohistochemical analysis of paraffin embedded Rat testis tissue labeling PLGF with ab196666 at 1/200 dilution. (400x magnification).
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Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue labeling PLGF with ab196666 at 1/200 dilution. (400x magnification).
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Immunohistochemical analysis of paraffin embedded Rat kidney tissue labeling PLGF with ab196666 at 1/200 dilution. (400x magnification).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab196666 has been referenced in 10 publications.
- Kim DK et al. PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141+ cancer-associated fibroblasts in pancreatic cancer. Nat Commun 13:6292 (2022). PubMed: 36272973
- Atzori MG et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor. Cancers (Basel) 14:N/A (2022). PubMed: 36428669
- Zhang W et al. PlGF knockdown attenuates hypoxia-induced stimulation of cell proliferation and glycolysis of lung adenocarcinoma through inhibiting Wnt/ß-catenin pathway. Cancer Cell Int 21:18 (2021). PubMed: 33407494
- Scaife PJ et al. Increased Placental Cell Senescence and Oxidative Stress in Women with Pre-Eclampsia and Normotensive Post-Term Pregnancies. Int J Mol Sci 22:N/A (2021). PubMed: 34298913
- Wang D et al. Involvement of Hdac3-mediated inhibition of microRNA cluster 17-92 in bronchopulmonary dysplasia development. Mol Med 26:99 (2020). PubMed: 33143661
- Gai S et al. Circular RNA hsa_circ_0007121 regulates proliferation, migration, invasion, and epithelial-mesenchymal transition of trophoblast cells by miR-182-5p/PGF axis in preeclampsia. Open Med (Wars) 15:1061-1071 (2020). PubMed: 33336062
- Al-Zeheimi N et al. Neoadjuvant Chemotherapy Alters Neuropilin-1, PlGF, and SNAI1 Expression Levels and Predicts Breast Cancer Patients Response. Front Oncol 9:323 (2019). PubMed: 31106153
- Vaquié A et al. Injured Axons Instruct Schwann Cells to Build Constricting Actin Spheres to Accelerate Axonal Disintegration. Cell Rep 27:3152-3166.e7 (2019). PubMed: 31189102
- Gimble JM et al. A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model. Plast Reconstr Surg Glob Open 6:e2010 (2018). PubMed: 30881803
- Naik A et al. Neuropilin-1 Associated Molecules in the Blood Distinguish Poor Prognosis Breast Cancer: A Cross-Sectional Study. Sci Rep 7:3301 (2017). IHC-P ; Human . PubMed: 28607365