Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
Database link: P49763
Our Abpromise guarantee covers the use of ab9542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 1 - 5 µg/ml.
Allows the detection of 1.0 - 2.5 ng/well of recombinant human PLGF-1 and PLGF-2.
|WB||Use a concentration of 1 - 4 µg/ml.
It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane.
|IHC-P||Use at an assay dependent concentration. PubMed: 19336420|
|ICC/IF||Use a concentration of 1 µg/ml.|
Western blot of chromatography purified, recombinant PlGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
PlGF antibody shows no cross-reactivity to VEGF165.