His-PLK1 full length purified from Sf9 cells.
Our Abpromise guarantee covers the use of ab17057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/200. PubMed: 19033445|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunofluoresence using ab17057 and either HeLaS3, NIH 3T3 or U2OS cells.
Western blot using ab17057.
Lane1: recombinant Plk1
Lane 2: U2OS cell extract
Lane 3: HeLaS3 cell extract
10% SDS-PAGE gel.
Overlay histogram showing U20S cells stained with ab17057 (red line). The cells were fixed with 80% methanol (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab17057, 1 µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 1 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.