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Recombinant full length protein (Xenopus laevis).
Our Abpromise guarantee covers the use of ab12212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 68 kDa).|
Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extract
AZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.
Julian Gannon, CRUK
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"