• Product nameAnti-PLK3 antibody
    See all PLK3 primary antibodies
  • Description
    Rabbit polyclonal to PLK3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PLK3.

    (Peptide available as ab33118.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Irradiated HeLa; MCF7; HEK293.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab33119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72, 75 kDa (predicted molecular weight: 72 kDa).


  • FunctionSerine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
  • Tissue specificityTranscripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CNK antibody
    • Cytokine Inducible Kinase antibody
    • Cytokine-inducible serine/threonine-protein kinase antibody
    • FGF inducible kinase antibody
    • FGF-inducible kinase antibody
    • FNK antibody
    • PLK-3 antibody
    • Plk3 antibody
    • PLK3_HUMAN antibody
    • Polo Like Kinase 3 antibody
    • Polo-like kinase 3 antibody
    • PRK antibody
    • Proliferation Related Kinase antibody
    • Proliferation-related kinase antibody
    • Serine/threonine-protein kinase PLK3 antibody
    see all

Anti-PLK3 antibody images

  • All lanes : Anti-PLK3 antibody (ab33119) at 1 µg/ml

    Lane 1 : Irradiated Hela Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 22 kDa,38 kDa,75 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
    We hypothesize that the 72 and 75 kDa bands represent the unmodified and phosphorylated forms of PLK3, respectively. This banding pattern of PLK3 is also observed in Bahassi et al., 2004. Oncogene 23, 2658–2663.
  • ICC/IF image of ab33119 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33119, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PLK3 antibody (ab33119)

This product has been referenced in:
  • Helmke C  et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res 26:914-34 (2016). Read more (PubMed: 27325299) »

See 1 Publication for this product

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