Overview

  • Product nameAnti-PLK3 antibody
    See all PLK3 primary antibodies
  • Description
    Rabbit polyclonal to PLK3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PLK3.

    (Peptide available as ab33118.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Irradiated HeLa; MCF7; HEK293.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72, 75 kDa (predicted molecular weight: 72 kDa).

Target

  • FunctionSerine/threonine protein kinase involved in regulating M phase functions during the cell cycle. May also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein.
  • Tissue specificityTranscripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CNK antibody
    • Cytokine Inducible Kinase antibody
    • Cytokine-inducible serine/threonine-protein kinase antibody
    • FGF inducible kinase antibody
    • FGF-inducible kinase antibody
    • FNK antibody
    • PLK-3 antibody
    • Plk3 antibody
    • PLK3_HUMAN antibody
    • Polo Like Kinase 3 antibody
    • Polo-like kinase 3 antibody
    • PRK antibody
    • Proliferation Related Kinase antibody
    • Proliferation-related kinase antibody
    • Serine/threonine-protein kinase PLK3 antibody
    see all

Anti-PLK3 antibody images

  • All lanes : Anti-PLK3 antibody (ab33119) at 1 µg/ml

    Lane 1 : Irradiated Hela Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 22 kDa,38 kDa,75 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab33119 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33119, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PLK3 antibody (ab33119)

ab33119 has not yet been referenced specifically in any publications.

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