Overview

  • Product nameAnti-PLK4 antibody
    See all PLK4 primary antibodies
  • Description
    Rabbit polyclonal to PLK4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human PLK4.

    (Peptide available as ab71393.)

  • Positive control
    • WB: HeLa, Jurkat, WI38, Raji, Ramos and MOLT4 whole cell lysates and testis tissue lysate. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 109 kDa).

Target

  • FunctionSerine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 1 POLO box domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated; leading to its degradation by the proteasome.
    Tyrosine-phosphorylated by TEC.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
  • Information by UniProt
  • Database links
  • Alternative names
    • PLK 4 antibody
    • PLK-4 antibody
    • PLK4 antibody
    • PLK4_HUMAN antibody
    • Polo like Kinase 4 antibody
    • Polo-like kinase 4 antibody
    • Protein serine/threonine kinase antibody
    • SAK antibody
    • Serine/Threonine Kinase 18 antibody
    • Serine/threonine kinase antibody
    • Serine/threonine protein kinase 18 antibody
    • Serine/threonine protein kinase PLK4 antibody
    • Serine/threonine protein kinase Sak antibody
    • Serine/threonine-protein kinase 18 antibody
    • Serine/threonine-protein kinase PLK4 antibody
    • Serine/threonine-protein kinase SAK antibody
    • Snk Akin Kinase antibody
    • STK 18 antibody
    • STK18 antibody
    see all

Anti-PLK4 antibody images

  • ICC/IF image of ab71394 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71394, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PLK4 antibody (ab71394) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human PLK4 peptide (ab71393) at 2 µg/ml
    Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human PLK4 peptide (ab71393) at 2 µg/ml
    Lane 10 : Human testis tissue lysate - total protein (ab30257) with Human PLK4 peptide (ab71393) at 2 µg/ml
    Lane 11 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate with Human PLK4 peptide (ab71393) at 2 µg/ml
    Lane 12 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate with Human PLK4 peptide (ab71393) at 2 µg/ml
    Lane 13 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate with Human PLK4 peptide (ab71393) at 2 µg/ml
    Lane 14 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate with Human PLK4 peptide (ab71393) at 2 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 109 kDa


    Exposure time : 2 minutes

    The 90 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to PLK4.

  • All lanes : Anti-PLK4 antibody (ab71394) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : NIH-3T3 cell lysate
    Lane 3 : Mouse testis lysate
    Lane 4 : Rat testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Swine anti-Rabbit HRP at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 109 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 7 minutes

    This image is courtesy of an Abreview submitted by Antibody Solutions

    See Abreview

References for Anti-PLK4 antibody (ab71394)

This product has been referenced in:
  • Bakre A  et al. Identification of Host Kinase Genes Required for Influenza Virus Replication and the Regulatory Role of MicroRNAs. PLoS One 8:e66796 (2013). Human . Read more (PubMed: 23805279) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Jurkat NIH-3T3 Ms Testis Rt Testis)
Loading amount 20 µg
Specification Jurkat NIH-3T3 Ms Testis Rt Testis
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 12 2011

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