Anti-PLOD1 antibody - N-terminal (ab171140)

Overview

  • Product name
    Anti-PLOD1 antibody - N-terminal
  • Description
    Rabbit polyclonal to PLOD1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PLOD1 aa 73-102 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q02809

  • Positive control
    • U251 cells and lysate; Human heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171140 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 84 kDa.

Target

  • Function
    Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
  • Involvement in disease
    Defects in PLOD1 are the cause of Ehlers-Danlos syndrome type 6 (EDS6) [MIM:225400]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS6 is characterized by the presence of ocular complications, particularly retinal detachment.
    Defects in PLOD1 are the cause of Nevo syndrome (NEVOS) [MIM:601451]. This is a rare, autosomal recessive disorder characterized by increased perinatal length, kyphosis, muscular hypotonia, and joint laxity. Nevo syndrome and EDS-VI have similar clinical phenotypes. Some authors consider that both syndromes are the same clinical entity.
  • Sequence similarities
    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization
    Rough endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-oxoglutarate 5-dioxygenase 1 antibody
    • EDS6 antibody
    • LH antibody
    • LH1 antibody
    • LLH antibody
    • Lysine hydroxylase antibody
    • Lysyl hydroxylase 1 antibody
    • PLOD antibody
    • Plod1 antibody
    • PLOD1_HUMAN antibody
    • Procollagen lysine 1 2 oxoglutarate 5 dioxygenase 1 antibody
    • Procollagen lysine 2 oxoglutarate 5 dioxygenase 1 antibody
    • Procollagen-lysine antibody
    see all

Images

  • Anti-PLOD1 antibody - N-terminal (ab171140) at 1/100 dilution + U251 cell line lysate at 35 µg

    Predicted band size: 84 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling PLOD1 with ab171140 at 1/10 diution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of U251 cells labeling PLOD1 with ab171140 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab171140 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Human Tissue sections (human liver)
Specification
human liver
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Jan 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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