• Product name
  • Description
    Mouse polyclonal to PLOD2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-R
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PLOD2 protein (NP_891988.1, 1 a.a. ~ 758 a.a)

  • Positive control
    • Human kidney tissue lysate. PLOD2 transfected 293T cell lysate. HeLa cells.



Our Abpromise guarantee covers the use of ab72939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
ICC/IF Use a concentration of 10 µg/ml.
  • Application notes
    Is unsuitable for IHC-R.
  • Target

    • Function
      Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
    • Tissue specificity
      Highly expressed in pancreas and muscle. Isoform 1 and isoform 2 are expressed in the majority of the examined cell types. Isoform 2 is specifically expressed in skin, lung, dura and aorta.
    • Involvement in disease
      Defects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2) [MIM:609220]. Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal.
    • Sequence similarities
      Contains 1 Fe2OG dioxygenase domain.
    • Cellular localization
      Rough endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2-oxoglutarate 5-dioxygenase 2 antibody
      • LH2 antibody
      • Lysine hydroxylase 2 antibody
      • Lysyl hydroxylase 2 antibody
      • OTTHUMP00000215204 antibody
      • OTTHUMP00000215205 antibody
      • OTTHUMP00000215206 antibody
      • PLOD 2 antibody
      • Plod2 antibody
      • PLOD2_HUMAN antibody
      • Procollagen lysine 2 oxoglutarate 5 dioxygenase 2 antibody
      • Procollagen lysine, 2 oxoglutarate 5 dioxygenase (lysine hydroxylase) 2 antibody
      • Procollagen-lysine antibody
      • Telopeptide lysyl hydroxylase antibody
      • TLH antibody
      see all

    Anti-PLOD2 antibody images

    • Anti-PLOD2 antibody (ab72939) at 1 µg/ml + human kidney tissue lysate at 25 µg

      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 85 kDa
      Observed band size : 85 kDa
    • All lanes : Anti-PLOD2 antibody (ab72939) at 1 µg/ml

      Lane 1 : PLOD2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 85 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
    • ab72939, at 10µg/ml, staining PLOD2 in Hela cells by immunofluorescence.

    References for Anti-PLOD2 antibody (ab72939)

    ab72939 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Resin sections)
    Human Tissue sections (Nasal polyp, tonsil)
    Nasal polyp, tonsil

    Abcam user community

    Verified customer

    Submitted Oct 12 2011


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