Anti-PLOD2/LH2 antibody (ab72939)
Key features and details
- Mouse polyclonal to PLOD2/LH2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLOD2/LH2 antibody
See all PLOD2/LH2 primary antibodies -
Description
Mouse polyclonal to PLOD2/LH2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PLOD2/LH2 aa 1-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_891988.1
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. -
Tissue specificity
Highly expressed in pancreas and muscle. Isoform 1 and isoform 2 are expressed in the majority of the examined cell types. Isoform 2 is specifically expressed in skin, lung, dura and aorta. -
Involvement in disease
Defects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2) [MIM:609220]. Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal. -
Sequence similarities
Contains 1 Fe2OG dioxygenase domain. -
Cellular localization
Rough endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5352 Human
- Omim: 601865 Human
- SwissProt: O00469 Human
- Unigene: 477866 Human
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Alternative names
- 2-oxoglutarate 5-dioxygenase 2 antibody
- LH2 antibody
- Lysine hydroxylase 2 antibody
see all
Images
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Anti-PLOD2/LH2 antibody (ab72939) at 1 µg/ml + human kidney tissue lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
All lanes : Anti-PLOD2/LH2 antibody (ab72939) at 1 µg/ml
Lane 1 : PLOD2/LH2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
ab72939, at 10µg/ml, staining PLOD2/LH2 in Hela cells by immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab72939 has been referenced in 3 publications.
- Ju HQ et al. A circRNA signature predicts postoperative recurrence in stage II/III colon cancer. EMBO Mol Med 11:e10168 (2019). PubMed: 31475771
- Kiyozumi Y et al. PLOD2 as a potential regulator of peritoneal dissemination in gastric cancer. Int J Cancer 143:1202-1211 (2018). PubMed: 29603227
- Shao S et al. Lysyl Hydroxylase Inhibition by Minoxidil Blocks Collagen Deposition and Prevents Pulmonary Fibrosis via TGF-ß1/Smad3 Signaling Pathway. Med Sci Monit 24:8592-8601 (2018). PubMed: 30481795