Overview

  • Product nameAnti-PLOD2 antibody
    See all PLOD2 primary antibodies
  • Description
    Rabbit polyclonal to PLOD2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 468-517 (KGKTLRSEMN ERNYFVRDKL DPDMALCRNA REMTLQREKD SPTPETFQML) of Human PLOD2 (NP_891988).

  • Positive control
    • DU145 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab90088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

  • FunctionForms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.
  • Tissue specificityHighly expressed in pancreas and muscle. Isoform 1 and isoform 2 are expressed in the majority of the examined cell types. Isoform 2 is specifically expressed in skin, lung, dura and aorta.
  • Involvement in diseaseDefects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2) [MIM:609220]. Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal.
  • Sequence similaritiesContains 1 Fe2OG dioxygenase domain.
  • Cellular localizationRough endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-oxoglutarate 5-dioxygenase 2 antibody
    • LH2 antibody
    • Lysine hydroxylase 2 antibody
    • Lysyl hydroxylase 2 antibody
    • OTTHUMP00000215204 antibody
    • OTTHUMP00000215205 antibody
    • OTTHUMP00000215206 antibody
    • PLOD 2 antibody
    • Plod2 antibody
    • PLOD2_HUMAN antibody
    • Procollagen lysine 2 oxoglutarate 5 dioxygenase 2 antibody
    • Procollagen lysine, 2 oxoglutarate 5 dioxygenase (lysine hydroxylase) 2 antibody
    • Procollagen-lysine antibody
    • Telopeptide lysyl hydroxylase antibody
    • TLH antibody
    see all

Anti-PLOD2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling PLOD2 with ab90088 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - PLOD2. Right - Merge.
  • Anti-PLOD2 antibody (ab90088) at 1 µg/ml (in 5% skim milk / PBS buffer) + DU145 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Gel concentration: 12%

References for Anti-PLOD2 antibody (ab90088)

ab90088 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"